Invited Speakers
Dr. Sevgi Gezici
Associate ProfessorFaculty of Medicine, Department of Medical Biology & Genetics, Gaziantep University, Turkey
Speech Title: Anthocyanin‑Rich Red and Purple Grape Suppress Cell Proliferation in Human Colon Cancers through Inducing Apoptosis
Abstract: Anthocyanins are natural compounds and produce phenolic acid metabolites that contribute to their antioxidative, anti-inflammatory, antiproliferative, and anti-carcinogenic properties. Previous reports have shown that an anthocyanins-rich diet helps to suppress cell proliferation, thereby preventing metastasis in colorectal cancer, among other types of cancer. Grape (Vitis vinifera L., Vitaceae) fruits, known to contain high amounts of anthocyanins, have are edible berry fruits that known to contain high amounts of anthocyanins, have wide range of biological activities and pharmacological properties. The objective of this research was to determine anticancer and antiproliferative effects of the grape juice obtained from red and purple grapes, and to reveal their mechanism of action by inducing apoptosis. Red grape juice (RGJ) and purple grape juice (PGJ) were prepared and semi-purified by solid-phase extraction (SPE). Sugars, acids, and other water-soluble compounds, and polyphenols (other than anthocyanins) were removed from the extracts. The purified extract was tested against human colon cancer cells, HT-29 and HCT-116, and normal human colon cells (CCD-33Co). Cell viability and anticancer effect of the juice extracts against the cancer cells were determined using MTT assay. The extracts were added in various concentrations (0.2-2.0 mg/mL), as well as doxorubicin (5–50 μM), and calculated IC50 values. Apoptotic bodies in human colorectal cancer cells treated with RGJ and PGJ were analyzed using the immunologic based ELISA method. Both of the grape juice was found to inhibit cell proliferation towards the tested colon cancer cells, with RGJ being the most potent inhibitor and the percentage of inhibition at 1 mg/mL against HT-29 and HCT-116 were 54.10% and 48.75%, respectively. Accordingly, this study reveals the anticancer effects of red and purple grape juice against human colon cancer cells by inducing apoptosis and suppressing cell proliferation.